منابع مشابه
A novel expression vector for the cyanobacterium, Synechococcus PCC 6301.
A cyanobacterial expression vector was constructed using ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) promoter and terminator sequences derived from Synechococcus PCC 6301. recombinant plasmid, designated pARUB19, has an ampicillin-resistant (ApR) gene as a selectable marker and four unique restriction sites to allow the insertion of foreign genes. Using this vector, the luciferase...
متن کاملEffect of heat shock on protein synthesis in the cyanobacterium Synechococcus sp. strain PCC 6301.
The response to heat shock at 47 degrees C was examined in the cyanobacterium (blue-green alga) Synechococcus sp. strain PCC 6301. On heat shock, the growth of the cells decreased and they preferentially synthesized a limited number of polypeptides. The rate of synthesis of these proteins increased markedly in the early period of temperature shift up and gradually decreased afterwards. Among th...
متن کاملNucleotide sequence of the psaE gene of cyanobacterium Synechococcus sp. PCC 6301.
The cyanobacterial PSI complex is a membrane-bound, photooxidoreductase that catalyzes the light-driven transport of electrons from reduced plastocyanin (or Cyt cSs3) to oxidized, soluble Fd or flavodoxin (for a review, see Golbeck and Bryant, 1991). The complex probably comprises single copies of 11 or 12 different polypeptides, about 100 Chl a molecules, 10 to 15 p-carotene molecules, 2 molec...
متن کاملStructural and regulatory properties of pyruvate kinase from the Cyanobacterium synechococcus PCC 6301.
Pyruvate kinase (PK) from the cyanobacterium Synechococcus PCC 6301 was purified 1,300-fold to electrophoretic homogeneity and a final specific activity of 222 micromol of pyruvate produced/min/mg of protein. The enzyme was shown to have a pI of 5.7 and to exist as a 280-kDa homotetramer composed of 66-kDa subunits. This PK appears to be immunologically related to Bacillus PK and a green algal ...
متن کاملNitrate transport and not photoinhibition limits growth of the freshwater Cyanobacterium synechococcus species PCC 6301 at low temperature.
The effect of low temperature on cell growth, photosynthesis, photoinhibition, and nitrate assimilation was examined in the cyanobacterium Synechococcus sp. PCC 6301 to determine the factor that limits growth. Synechococcus sp. PCC 6301 grew exponentially between 20 degreesC and 38 degreesC, the growth rate decreased with decreasing temperature, and growth ceased at 15 degreesC. The rate of pho...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1984
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-130-11-2771